| No. | Pos. | Player | DoB/Age | Caps | Club |
|---|
| 1 | GK | Iker Casillas (c) | 20 May 1981 (aged 29) | 104 |  Real Madrid |
| 2 | DF | Raúl Albiol | 4 September 1985 (aged 24) | 23 | Real Madrid |
| 3 | DF | Gerard Piqué | 2 February 1987 (aged 23) | 16 | Barcelona |
| 4 | DF | Carlos Marchena | 31 July 1979 (aged 30) | 59 | Valencia |
| 5 | DF | Carles Puyol | 13 April 1978 (aged 32) | 83 | Barcelona |
| 6 | MF | Andrés Iniesta | 11 May 1984 (aged 26) | 43 | Barcelona |
| 7 | FW | David Villa | 3 December 1981 (aged 28) | 58 | Valencia |
| 8 | MF | Xavi | 25 January 1980 (aged 30) | 87 | Barcelona |
| 9 | FW | Fernando Torres | 20 March 1984 (aged 26) | 73 |  Liverpool |
| 10 | MF | Cesc Fàbregas | 4 May 1987 (aged 23) | 49 | Arsenal |
| 11 | DF | Joan Capdevila | 3 February 1978 (aged 32) | 46 | Villarreal |
| 12 | GK | Víctor Valdés | 14 January 1982 (aged 28) | 1 | Barcelona |
| 13 | MF | Juan Manuel Mata | 28 April 1988 (aged 22) | 8 | Valencia |
| 14 | MF | Xabi Alonso | 25 November 1981 (aged 28) | 69 | Real Madrid |
| 15 | DF | Sergio Ramos | 30 March 1986 (aged 24) | 60 | Real Madrid |
| 16 | MF | Sergio Busquets | 16 July 1988 (aged 21) | 13 | Barcelona |
| 17 | DF | Álvaro Arbeloa | 17 January 1983 (aged 27) | 15 | Real Madrid |
| 18 | FW | Pedro | 28 July 1987 (aged 22) | 3 | Barcelona |
| 19 | FW | Fernando Llorente | 26 February 1985 (aged 25) | 7 | Athletic Bilbao |
| 20 | MF | Javi Martínez | 2 September 1988 (aged 21) | 2 | Athletic Bilbao |
| 21 | MF | David Silva | 8 January 1986 (aged 24) | 36 | Valencia |
| 22 | MF | Jesús Navas | 21 November 1985 (aged 24) | 6 | Sevilla |
| 23 | GK | Pepe Reina | 31 August 1982 (aged 27) | 20 | Liverpool |
1
Netherlands
1
Germany
Uruguay
Brazil
Italy
Portugal
Argentina
Croatia
Chile
Norway
Russia
Greece
Côte d'Ivoire
France
Japan
Sweden
Montenegro
Mexico
Denmark
Australia
Slovenia
Turkey
Paraguay